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Image Search Results
Journal: Blood
Article Title: Effects of intravenous immunoglobulin on platelet count and antiplatelet antibody disposition in a rat model of immune thrombocytopenia
doi: 10.1182/blood.v100.6.2087
Figure Lengend Snippet: Figure 3. IVIG does not directly bind 7E3. 7E3 (f) (or control IgG, ) and IVIG were combined in vitro, at a constant IVIG concentration (25 mg/mL) and varying 7E3 concentrations (0-0.1 mg/mL). The positive control was a mouse antihuman IgG. Samples were then added to a microplate coated with antihuman IgG. Murine IgG binding was visualized using a secondary antimouse IgG–alkaline phosphatase conjugate. p-Nitrophenyl phosphate was added, and the plates were read at 405 nm (kinetic assay, over 10 minutes). Assay response to 7E3 did not differ from control (P .164), whereas the positive control differed significantly from control (P .001).
Article Snippet: Goat antihuman IgG (no cross-reactivity to goat and mouse serum proteins) and
Techniques: Control, In Vitro, Concentration Assay, Positive Control, Binding Assay, Kinetic Assay